PRODUCTS AND SERVICES

Scaffold ssDNA for DNA origami

We offer a range of single-stranded scaffold DNA for DNA origami nanostructures, varying in length and sequence. Our focused portfolio allows us to maintain high quality standards across our products. Each unit of scaffold DNA typically supports between 25 to 400 standard DNA origami folding reactions (20 nM, 100 µl).

Buy now

Why order with us?

  • Quick delivery times

    Get your ssDNA fast. We prioritize quick delivery times worldwide, with shipping out within 2-3 workdays while understanding what's required for compliance with all customs regulations.

  • In-house specialists

    Our DNA origami experts and biotechnologists are available at any time. Receive help with trouble shooting and get advice on the quantities or best sequence length.

  • Specialized on DNA Origami

    Our deep expertise in DNA origami enables us to provide scaffold DNA optimized for your research needs. Through direct customer engagement and our own hands-on experience, we continually develop products that enhance the capabilities of DNA origami design.

For DNA Origami Experts by DNA Origami Experts and Biotechnologists

At our core, we are DNA origami experts, working daily with the very scaffolds we provide. With more than a decade of experience in producing and shipping high-quality scaffolds, we understand the unique challenges and demands of DNA origami research. Our team, made up of seasoned scientists and biotechnologists, continuously innovates in scaffold production, refining both our processes and scaffold types to stay ahead of the curve.

When you work with us, you're not just purchasing a product—you're gaining direct access to experts who are ready to answer your questions and support your research.

Quality control is at the heart of everything we do. We conduct thorough QC on all of our single-stranded DNA (ssDNA) products, ensuring that you can trust their integrity and focus entirely on your experimental goals without the worry of subpar materials.

Original sequence of the M13mp18 phage

Our scaffolds are produced by extension of the ssDNA of the filamentous M13mp18 phage through molecular cloning. This allows us to produce scaffolds with various lenghts. We provide the most commonly used lengths for DNA Origami in different volumes

Shortened and extended variants of M13mp18

Our shortened scaffolds, like the 4,844 base pair version, are perfect for creating compact, efficient DNA origami structures. By reducing complexity, they allow for faster folding and more precise designs, giving you greater control over your experiments.

Our extended scaffolds (7,560 and 8,064 base pairs) offer the flexibility to build larger, more intricate designs.

We can provide shorter scaffolds by cleaving on request.

Orthogonal scaffold

Our orthogonal scaffolds allow you to assemble multiple separate nanostructures simultaneously in a single one-pot reaction, or to create larger, more intricate designs that incorporate multiple scaffold strands.

Their sequences do not interact with the standard scaffolds (based on M13mp18) or exhibit minimal cross-reactivity due to their distinct sequences, making them perfect for advanced DNA origami applications.

Whether you're looking to streamline your experimental process or design highly complex nanostructures, our orthogonal scaffolds provide the flexibility and innovation to bring your most ambitious projects to life.

Buy nowRequest a quote

Examples of publications with our scaffold

  1. Hu, Y., Rogers, J., Duan, Y. et al. Quantifying T cell receptor mechanics at membrane junctions using DNA origami tension sensors. Nat. Nanotechnol. (2024). https://doi.org/10.1038/s41565-024-01723-0
  2. Ober, M.F., Baptist, A., Wassermann, L. et al. In situ small-angle X-ray scattering reveals strong condensation of DNA origami during silicification. Nat Commun 13, 5668 (2022). https://doi.org/10.1038/s41467-022-33083-5 
  3. Xin, L., Zhou, C., Duan, X. et al. A rotary plasmonic nanoclock. Nat Commun 10, 5394 (2019). https://doi.org/10.1038/s41467-019-13444-3
  4. Zanacchi, F., Manzo, C., Alvarez, A. et al. A DNA origami platform for quantifying protein copy number in super-resolution. Nat Methods 14, 789–792 (2017). https://doi.org/10.1038/nmeth.4342

Frequently Asked Questions

Ordering from China or Japan?

You can order our ssDNA in China through Biohub International Trade and MBL Biotech
In Japan directly with us or through Eurofins Genomics

How is tilibit ssDNA for DNA origami delivered?

Your single-stranded DNA is delivered in a specific buffer at a specific concentration.

What is the best way to store and handle ssDNA for DNA origami

ssDNA stability is affected by both the buffer used and the temperature at which it is stored. For extended preservation, it is best stored at -20 °C, whereas for temporary storage, 4 °C is advisable.

Avoid shearing, pipette gently.

How do I determine the correct ssDNA type for my DNA origami project?

The ordering type and length depend on your specific DNA origami design. We recommend  contacting us to advise you in the best fitting product for your design.

Are the scaffold sequences identical to the sequences supplied with the cadnano software?

The sequence of the single-stranded scaffold DNA, type p7249 is identical to the sequence "M13mp18" in cadnano.

The sequence of the single-stranded scaffold DNA, type p7560 is identical to the sequence "p7560" in cadnano.

The sequence of the single-stranded scaffold DNA, type p8064 is identical to the sequence "p8064" in cadnano.

Are there higher concentrations of ssDNA available?

Solutions with single-stranded scaffold DNA concentrations above 400 nM are viscose and difficult to handle. Please contact us if your application requires higher concentration and we will find a solution that works for your project.